Solubilization of chloroplast membranes by zwitterionic detergents
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چکیده
منابع مشابه
Solubilization of human erythrocyte membranes by ASB detergents.
Understanding the membrane solubilization process and finding effective solubilizing agents are crucial challenges in biochemical research. Here we report results on the interaction of the novel linear alkylamido propyl dimethyl amino propanosulfonate detergents, ASB-14 and ASB-16, with human erythrocyte membranes. An estimation of the critical micelle concentration of these zwitterionic deterg...
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Neurotoxic esterase (NTE) is a membrane-bound protein found in highest concentration in brain and lymphocytes. The enzyme has no known physiological function, but its organophosphorylation and aging in neural tissue are thought to trigger the pathogenesis of organophosphorus-induced delayed neuropathy (OPIDN). Solubilization of NTE from microsomal membranes from hen or chick brain was studied w...
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enzymes is due to a more specific process than generalizedcell damage or loss (Holdsworth & Coleman, 1975). We have now attempted to make an initial separation of these enzymes from other biliary proteins, before investigating their relationship with the bile-canaliculus region of the hepatocyte plasma membrane. Fresh sheep bile was treated with solid protamine sulphate and centrifuged to remov...
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The glycolate/glycerate transporter of spinach (Spinacia oleracea L.) chloroplast inner envelope membranes was solubilized by treatment of the membranes with sodium cholate. Mixtures of the cholate extracts and soy asolectin were subjected to gel filtration to remove the detergent. The reconstituted vesicles were frozen, thawed, and sonicated in a buffer that contained 10 millimolar d-glycerate...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1979
ISSN: 0014-5793
DOI: 10.1016/0014-5793(79)80237-9